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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LEAP2
Then Streptavidin-HRP is added and binds to the Biotinylated AHSG antibody
And then biotinylated Human EFNB1 Antibody is added and binds to EFNB1 in the sample
The assay plate has been pre-coated with mouse anti-Human CTLA-4 monoclonal antibody
a horseradish peroxidase conjugated mouse anti-Human CD55 Antibody is added to the wells and binds to CD55 in the sample
Mouse HMGB-1 (High Mobility Group Protein B1) ELISA Kit Packaging:96 wells The microtiter plate provided inThe Mouse (HMGB 1) High Mobility Group Protein B1 ELISA Kit measures High Mobility Group Protein B1 in Mouse samples. The plate has been pre coated with Mouse HMGB1 antibody. HMGB1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse HMGB1 Antibody is added and binds to HMGB1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated HMGB1 antibody. After incubation unbound
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