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Then Streptavidin-HRP is added and binds to the Biotinylated LRAT antibody
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat sRANKL
coli expression system and the target gene encoding Ala26-Arg221 is expressed with a 6His tag at the N-terminus
The plate has been pre-coated with Mouse LIF antibody
Then a biotinylated detection antibody specific for Mouse GROBeta/CXCL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human NRK (Nik-related Protein Kinase) ELISA Kit IL-6R-1 Then Streptavidin-HRP is added andThe Human (NRK) Nik related Protein Kinase ELISA Kit measures Nik Related Protein Kinase in samples. The plate has been pre coated with Human NRK antibody. NRK present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NRK Antibody is added and binds to NRK in the sample. Then Streptavidin HRP is added and binds to the Biotinylated NRK antibody. After incubation unbound Streptavidin HRP is washed away
