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And then biotinylated Rat CNTF Antibody is added and binds to CNTF in the sample
Then a biotinylated detection antibody specific for Human OPG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Cost: Economical
The concentration of Mouse CYP27B1 in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Human MPIF-1/CCL23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Rat IL-4 (Interleukin 4) ELISA Kit Packaging:96 wells And then biotinylated Rat CNTFThe Rat (IL 4) Interleukin 4 ELISA Kit measures Interleukin 4 in Rat samples. The plate has been pre coated with Rat IL4 antibody. IL4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat IL4 Antibody is added and binds to IL4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IL4 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
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